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F-actin distribution is altered in apt1 mutant cells. Cells of the WT (H99) and apt1 mutant strains were grown to mid-log phase in YPD medium at 30°C. After harvest, the cells were fixed and stained with Alexa Fluor 568 phalloidin and DAPI. One hundred budded cells for each strain were examined using fluorescence microscopy and scored for actin distribution in buds. Bar = 10 μM.
