FIG. 1.

Accumulation of TAGs in lipid droplets during N limitation. (A) Quantification of total cellular fatty acids (black bars) and fatty acids in TAG (gray bars) of dw15 cells grown in medium with 10 mM NH₄⁺ (+N), or cells switched (arrow) to medium with no NH₄⁺ (−N) and grown for 24, 48, or 72 h. (B) Confocal microscopy images of Nile red-stained CC-125 cells grown in medium with 10 mM NH₄⁺ or cells switched (arrow) to medium with 0 mM NH₄⁺ and grown for 12, 24, or 48 h. Differential interference contrast (D), chlorophyll autofluorescence (C), Nile red fluorescence indicating lipid droplets (L), and all three images merged (M) are shown. The scale bars indicate 5 μm.