FIG. 3.
Characteristics of isolated lipid droplets. (A) Absorbance spectra of ethanol extracts (96%) normalized on an equal fatty acid basis from thylakoids of cells grown in medium containing 10 mM NH4+ (+N) or cells switched to 0 mM NH4+ and grown for 1 day (−N 1d) from enriched eyespot fraction and from lipid droplets were recorded. (B) Ratios of fatty acids derived from TAG (TAG FA) to total fatty acids (Total FA) for extracts from whole cells grown in medium with 10 mM NH4+ (+N) or whole cells switched to and grown in medium with no NH4+ (−N) for 24, 48, or 72 h, the enriched eyespot fraction (Eye), and isolated lipid droplets (LD) as indicated. (C) Proteins (30 μg) separated by gradient (4 to 20%) SDS-PAGE from whole cells (Tot. Prot., total protein), thylakoids, eyespots, and lipid droplets as indicated. The positions of molecular mass markers in kDa are indicated on the left, and sections excised from the gel separating lipid droplet-associated protein are indicated on the right.