FIG. 5.

Northern blot analysis of MLDP. Five micrograms of total RNA isolated from cells grown in 10 mM NH₄Cl (10 mM N) and cells deprived of N for 2 days (−N 2 d) was processed for Northern blotting as previously described (23). MLDP mRNA was specifically detected using a ³²P-radiolabeled probe amplified from cDNA with forward and reverse primers 5′-ATGGTGGTGATCTTGTTTGACAGAG-3′ and 5′-CATTCCGCTGTACCTCCAGG-3′. Total RNA was stained with methylene blue (23) and shown as a loading control (rRNA). The results for two biological replicates of this experiment are shown (panels 1 and 2).