TABLE 1.
Total fatty acid content of whole cells compared to fatty acids bound in TAGs isolated from +N cells and −N cellsb
| Fatty acid | Fatty acid content ofa: |
|||
|---|---|---|---|---|
| Whole cells |
TAG |
|||
| +N | −N | +N | −N | |
| 16:0 | 22.1 ± 0.14 | 26.2 ± 0.70 | 51.3 ± 1.30 | 37.6 ± 3.99 |
| 16:1 | 3.4 ± 0.02 | 2.9 ± 0.01 | 5.4 ± 2.37 | 3.7 ± 0.54 |
| 16:2 | 1.2 ± 0.00 | 1.4 ± 0.06 | 1.6 ± 0.96 | 1.4 ± 0.17 |
| 16:3 | 4.5 ± 0.15 | 3.4 ± 0.25 | 13.9 ± 6.88 | 3.2 ± 0.27 |
| 18:0 | 2.4 ± 0.02 | 2.5 ± 0.06 | 0.8 ± 0.84 | 1.4 ± 0.17 |
| 18:1, 16:4 | 21.9 ± 0.13 | 21.2 ± 0.14 | 7.9 ± 2.46 | 22.1 ± 8.45 |
| 18:2 | 8.0 ± 0.02 | 9.9 ± 0.06 | 6.1 ± 3.33 | 13.6 ± 1.51 |
| 18:3Δ5,9,12 | 6.7 ± 0.01 | 6.5 ± 0.12 | 4.6 ± 1.62 | 5.7 ± 0.63 |
| 18:3Δ9,12,15 | 27.9 ± 0.10 | 24.7 ± 0.13 | 7.3 ± 3.69 | 9.9 ± 1.20 |
| 18:4 | 1.9 ± 0.02 | 1.5 ± 0.02 | 1.1 ± 0.16 | 1.3 ± 0.16 |
a
Averages of three replicates and the standard deviation are shown.
b
+N indicates cells grown in medium containing 10 mM NH4+; −N indicates cells switched to 0 mM NH4+ and grown for 1 day.