TABLE 2.
Fatty acid content of whole organelles (eyespot and lipid droplet) compared to fatty acids bound in TAGs isolated from indicated organelles
| Fatty acid | Fatty acid content ofa: |
|||
|---|---|---|---|---|
| Eyespot |
Lipid droplet |
|||
| Whole organelle | TAG | Whole organelle | TAG | |
| 16:0 | 35.5 ± 0.67 | 67.0 ± 7.55 | 32.2 ± 1.24 | 41.8 ± 4.33 |
| 16:1 | 3.0 ± 0.01 | 4.5 ± 1.08 | 3.4 ± 0.37 | 3.1 ± 0.58 |
| 16:2 | 3.4 ± 0.02 | 3.0 ± 0.30 | 1.2 ± 0.15 | 0.9 ± 0.06 |
| 16:3 | 15.7 ± 0.18 | 7.8 ± 0.45 | 6.4 ± 3.03 | 5.1 ± 1.70 |
| 18:0 | 3.0 ± 0.01 | 1.9 ± 0.50 | 2.1 ± 0.87 | 1.5 ± 0.77 |
| 18:1, 16:4 | 18.4 ± 0.39 | 7.9 ± 1.99 | 27.2 ± 1.74 | 27.3 ± 2.86 |
| 18:2 | 10.9 ± 0.71 | 4.7 ± 1.77 | 10.7 ± 0.67 | 10.0 ± 1.67 |
| 18:3Δ5,9,12 | 5.6 ± 0.09 | 1.4 ± 0.57 | 7.7 ± 1.59 | 5.9 ± 2.62 |
| 18:3Δ9,12,15 | 3.3 ± 0.47 | 0.8 ± 0.41 | 6.8 ± 4.69 | 2.9 ± 0.34 |
| 18:4 | 1.1 ± 0.02 | 1.1 ± 0.47 | 2.3 ± 0.92 | 1.4 ± 0.38 |
a
Organelles were isolated from cells that were switched to medium containing 0 mM NH4+ and grown for 1 day. Two replicates were averaged for eyespot samples and three for lipid droplet samples. The averages and the standard deviation are shown.