Figure 4.

Crk SH3-domain binding proteins C3G, DOCK180 and Sos activate JNK. COS cells were transfected with pSRα3-(HA)JNK1 (JNK) and pCAGGS-expression vector coding for either DOCK180, Sos or C3G, with or without simultaneous wild-type c-Crk II expression. JNK kinase activity was measured as above by immunocomplex kinase assay, and the results are expressed as fold-induction compared with the JNK kinase activity observed in cells transfected with the pSRα3-(HA)JNK1-vector and an empty pCAGGS-vector.