FIG. 2.

T. brucei ribosomal DNA transcription units show relative depletion of histone H3 around the promoter regions. (A) Schematic depicting the region around a T. brucei rDNA transcription unit according to White et al. (80); the rDNA promoter is indicated by a flag, the rDNA genes are shown as black boxes, and the regions analyzed by qPCR are indicated by lettered brackets. (B) Distribution of histone H3 over the rDNA as determined by ChIP using a histone H3 antibody (or no antibody as a negative control) in bloodstream form (BF) or procyclic form (PF) T. brucei. qPCR was used to amplify regions indicated in panel A. Signals are expressed as the percentage of input immunoprecipitated after subtraction of signal from the no-antibody control. Results show the average from three independent experiments, with the standard deviations indicated with error bars.