PCR-based determination of MAT locus idiomorphs of M. gypseum. A 472-bp portion of the alpha domain MAT1-1 gene was obtained from strains CBS118893, ATCC 24164, ATCC 48981, ATCC 58593, ATCC 58595, and ATCC 24165 and progeny P1, P3, P5, P6, P8, and P10 of strains ATCC 24164 and ATCC 48982. A 502-bp portion of the HMG domain MAT1-2 gene was obtained from strains ATCC 24163, ATCC 48982, ATCC 24102, ATCC 24166 and the progeny P2, P4, P7, and P9 of strains ATCC 24164 and ATCC 48982. NC, negative control; M, DNA ladder marker.