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. 2009 Oct 30;9(1):46–58. doi: 10.1128/EC.00259-09

FIG. 7.

FIG. 7.

Virulence tests of M. gypseum in G. mellonella. (A) Twelve wax moth larvae were placed on Sabouraud dextrose agar plates on which M. gypseum strains were cultivated and surface infected by contact with the fungus. A Sabouraud dextrose agar plate without any M. gypseum served as the negative control. (B) G. mellonella larvae that turned dark and were nonresponsive to touch were scored as dead and removed. (C) The curves show survival of larvae during a 12-day period. By statistical analysis, M. gypseum strains ATCC 24102 (−), ATCC 24166 (−), ATCC 58593 (+), and ATCC 58595 (+) are not significantly virulent (P > 0.05). The remaining six strains CBS118893 (+), ATCC 48981 (+), ATCC 24164 (+), ATCC 24165 (+), ATCC 24163 (−), and ATCC 48982 (−) showed significant virulence (P < 0.001). No significant virulence difference was found between the mating type (+) and mating type (−) strains in the virulent group (P > 0.05). Our results suggest the two mating type strains of M. gypseum may have equivalent virulence in G. mellonella.