TABLE 2.

Primer pairs used for amplification of MAT and flanking regions and mating type determination of M. gypseum

Primer 1	Primer 1 sequence (5′-3′)	Primer 2	Primer 2 sequence (5′-3′)	Amplicon size (bp)a	Gene amplified	Aim
JOHE20209	CGAGAGCAACGACGTTCATA	JOHE20213	AACAACCGTTGGACAGAAGG	11,048	Unknown gene, APN2, COX13, and SLA2	Amplification of MAT locus and flanking region
JOHE20210	GATTGCCCGTTCTCTCTCAG	JOHE20211	GCATAACTGGCACCCTTTGT	5,910	Alpha box and APN2	Amplification of MAT locus and flanking region
JOHE20463	CACTGGCGAAAGTTATGCAA	JOHE20464	GCCAGCCAACCTTTCACTTA	5,549	COX13, and SLA2	Amplification of MAT locus and flanking region
JOHE20465	CACTCGGGCTTCGAGTAAAC	JOHE20466	TCTGGTCTGGCATGAAAGTG	5,658	APN2, COX13, and SLA2	Amplification of MAT locus and flanking region
JOHE20887	CCTCTTCTACTGCCATGACA	JOHE20888	CCATGGGATTGATGTGTGCA	452	MAT1-1 alpha domain	Mating type assignment
JOHE20893	GGATGAGTCCTGATATGTCA	JOHE20894	CCTATGGGTTTAGCTTCTGA	502	MAT1-2 HMG domain	Mating type assignment
JOHE21325	GCTATTGGTCGAGATAACGTCTTT	JOHE21326	TTAATCAATAACTGACCTGCTCCA	624	Polysaccharide export protein	Recombination test
JOHE21327	TGCTGTCCGTAAGTGTAGGTATGT	JOHE21328	CCAGCCTTGCTCTCAAGTAGTTAT	889	DNJ-oxidoreductase intergenic spacer	Recombination test

^aAmplicon sizes were deduced from the genome sequence of strain CBS118893 (Broad Institute) and the MAT sequence of strain ATCC 48982 (this study).