TABLE 3.
Mating of M. gypseum produces recombinant F1 progenya
| Strain | CAP59 genotype | DNJ-oxidoreductase intergenic spacer genotype | Mating typeb | Recombination |
|---|---|---|---|---|
| Parental strain | ||||
| ATCC 24164 | 1 | 1 | MAT1-1 | |
| ATCC 48982 | 2 | 2 | MAT1-2 | |
| F1 progeny | ||||
| P1 | 1 | 2 | MAT1-1 | Yes |
| P2 | 2 | 2 | MAT1-2 | No (ATCC 48982) |
| P3 | 2 | 2 | MAT1-1 | Yes |
| P4 | 2 | 2 | MAT1-2 | No (ATCC 48982) |
| P5 | 1 | 2 | MAT1-1 | Yes |
| P6 | 1 | 2 | MAT1-1 | Yes |
| P7 | 1 | 1 | MAT1-2 | Yes |
| P8 | 2 | 1 | MAT1-1 | Yes |
| P9 | 1 | 2 | MAT1-2 | Yes |
| P10 | 2 | 2 | MAT1-1 | Yes |
a
Genetic recombination of the F1 progeny of ATCC 24164 × ATCC 48982 was determined by sequencing two genetic markers, CAP59 and the DNJ-oxidoreductase intergenic spacer and by mating type PCR tests.
b
The mating types were identified by PCR (see Fig. 5).