TABLE 5.

Flagellar gene expression of L. pneumophila ΔrpoN, ΔfleQ, ΔfleR, and ΔfliA mutants in the late replicative and transmissive phases in BCYE

Gene name	Gene no.	Class	Relative mutant/WT gene expression ina:
			Replicative phase			Transmissive phase
			ΔrpoN/WT	ΔfleQ/WT	ΔfleR/WT	ΔrpoN/WT	ΔfleQ/WT	ΔfleR/WT	ΔfliA/WT
rpoN	lpp0542	I	0.104	—	—	0.061	—	—	—
fleQ	lpp0915	I	—b	0.216	—	2.138	0.364	—	1.436
fliJ	lpp1720	IIa	—	—	—	—	—	—	—
fliI	lpp1721	IIa	—	—	—	—	—	—	—
fliH	lpp1722	IIa	—	0.403	—	—	—	—	—
fliG	lpp1723	IIa	—	0.384	0.542	—	—	—	—
fliF	lpp1724	IIa	—	—	—	—	—	—	—
fliE	lpp1725	IIa	—	0.532	0.303	—	—	0.357	—
fleR	lpp1726	IIa	—	0.544	0.102	—	—	0.150	—
fleS	lpp1727	IIa	—	0.549	—	—	—	—	—
flhA	lpp1749	IIa	0.299	0.330	1.672	—	—	—	—
flhB	lpp1750	IIa	—	—	—	—	—	—	—
fliR	lpp1751	IIa	0.295	0.438	—	—	—	—	—
fliQ	lpp1752	IIa	0.392	0.382	—	—	—	—	—
fliP	lpp1753	IIa	0.306	0.382	1.695	—	—	—	—
fliO	lpp1754	IIa	0.174	0.243	—	—	—	—	—
fliN	lpp1755	IIa	0.060	0.095	4.015	0.446	0.456	1.878	—
fliM	lpp1756	IIa	0.058	0.113	2.362	0.278	0.335	2.220	—
flgA	lpp0970	IIb	0.605	—	—	—	—	—	—
flgB	lpp1224	IIb	0.212	0.363	—	0.357	—	—	—
flgC	lpp1225	IIb	0.384	0.536	—	—	—	—	—
flgD	lpp1226	IIb	0.403	0.470	1.684	—	—	2.564	—
flgE	lpp1227	IIb	—	0.304	—	—	0.231	3.998	—
flgF	lpp1228	IIb	—	0.215	2.499	—	0.429	5.280	—
flgG	lpp1229	IIb	0.262	0.279	1.865	—	—	3.023	—
flgH	lpp1230	IIb	0.376	0.556	1.752	—	0.526	2.965	—
flgI	lpp1231	IIb	0.310	0.177	1.783	—	—	2.662	—
flgJ	lpp1232	IIb	0.403	0.285	1.963	—	0.534	2.436	—
flgK	lpp1233	IIb	—	0.275	2.301	—	0.339	3.915	0.532
flgL	lpp1234	IIb	—	0.441	—	—	0.486	2.219	0.379
fliK′	lpp1657	IIb	—	—	—	—	—	—	—
fleN	lpp1747	IIb	0.118	0.157	1.993	0.233	0.362	2.604	—
flhF	lpp1748	IIb	0.102	0.147	1.834	0.308	0.435	—	—
flgN	lpp0968	III	—	—	—	—	0.420	—	0.068
flgM	lpp0969	III	—	—	—	—	0.309	—	0.081
motB	lpp1744	III	—	0.497	—	—	0.459	—	—
motA	lpp1745	III	—	0.606	—	—	—	1.922	0.052
fliA	lpp1746	III	—	0.457	—	—	0.484	2.587	0.042
motA2	lpp2266	III	1.849	0.583	—	2.545	0.521	—	—
motB2	lpp2267	III	—	—	—	1.864	—	1.950	—
flhB′	lpp2635	III	—	—	—	2.827	—	1.593	0.295
fliS	lpp1291	IV	—	0.643	—	—	0.399	—	0.048
fliD	lpp1292	IV	—	—	—	2.548	0.555	2.135	0.045
flaG	lpp1293	IV	—	—	0.527	—	0.212	—	0.007
flaA	lpp1294	IV	—	—	0.189	—	0.176	0.536	0.003
motY	lpp3034	IV	—	—	—	—	—	—	0.116

^aValues in boldface show statistically significant altered expression compared to that of the wild type, with a fold change of ≥2 or ≤0.5 at P ≤ 0.001. Values not in boldface show statistically significant altered expression compared to that of the wild type at P ≥ 0.001.

^b—, show no statistically significant gene expression changes.