FIG. 1.

RDA with C. fetus subsp. venerealis DNA as tester DNA reveals candidate clones for subspecies-specific genomic fragments. Typical results for the analysis are illustrated with duplicate dot blots of 71 clones. Blot A was hybridized with Sau3A1-digested and ³²P-labeled genomic C. fetus subsp. venerealis ATCC 19438 DNA. Blot B was hybridized with an equivalently prepared C. fetus subsp. fetus ATCC 27374 genomic DNA probe. The squares indicate clones specific for the C. fetus subsp. venerealis reference strain (no hybridization with the C. fetus subsp. fetus probe). DNA (500 ng) from reference strains were applied as positive controls (Cff and Cfv) as marked, and vector DNA (300 ng) was used to control for plasmid hybridization (pB).