TABLE 3.
Plasmids used in this study
| Plasmid | Relevant features | Reference or source |
|---|---|---|
| pBluescript II KS(−) | bla lacZ | Stratagene |
| pBlueKS−/VirB9 | virB9 cloned in the MCSa of pBluescript II KS(−) | This study |
| pGG5 | aphA-3 from pILL600 inserted in virB9 of pBlueKS−/VirB9 | This study |
| pILL600 | Source of aphA-3 | 52 |
| pRYVL1 | Φ (PsapA-aphA-3) in pRYGG1, BamHI, and PstI linker between PsapA and aphA-3 | 48 |
| pRYBM3 | Φ (PrpsJ-aphA-3); PsapA of pRYVL1 replaced by PrpsJ | This study |
| pRYBM5 | Φ (PgatC-aphA-3); PsapA of pRYVL1 replaced by PgatC | This study |
| pBlue-oriT | mobIncP of pILL570 in the pBluescript II KS(−) MCS | 48 |
| pSK5 | virD4 cloned into pBlue-oriT | This study |
| pSK6 | virD4 of pSK5 interrupted with PrpsJ-aphA-3 of pRYBM3 | This study |
| pBlue-oriT-cdt | cdtB cloned in pBlue-oriT | This study |
| pJL1 | cdtB of pBlue-oriT-cdt interrupted with Φ(PgatC-aphA-3) | This study |
| pRYSK8 | Φ (PT4SS-1.2-aphA-3); PsapA of pRYVL1 replaced by PT4SS-1.2 | This study |
| pRYSS1 | Φ (PsapA-aphA-3) in pRYGG1 | 48 |
| pRYVL2 | Φ (PsapA-virD4-aphA-3) in pRYGG1 | 48 |
a
MCS, multiple-cloning site.