TABLE 4.
Gene fragments identified during RDA and their annotation
| Clone | Best match (accession no.) | Length (nt) | % Identitya | % Similaritya | E value | No. of clones obtained |
Subspecies distribution (no./total) in Southern analysisb |
||
|---|---|---|---|---|---|---|---|---|---|
| 1st RDA | 2nd RDA | Cfv | Cff | ||||||
| 4Ch | GDP-mannose 4,6-dehydratase; Thiomicrospira crunogena XCL-2 (ABB42272.1) | 419 | 65 | 80 | 2 × 10−47 | 5 | 0 | 0/10 | 8/10 |
| 3Dv | Hypothetical protein pTet_29; Campylobacter jejuni 81-176 (YP_247557.1) | 354 | 42 | 56 | 9 × 10−10 | 0 | 1 | 10/10 | 0/10 |
| 3Cp | Flagellin B; Campylobacter fetus subsp. fetus 82-40 (ZP_01073199.1) | 366 | 70 | 86 | 1 × 10−23 | 4 | 6 | 9/10 | 2/10 |
| 1Au | TrbE; Agrobacterium tumefaciens (NP_059757.1) | 571 | 40 | 60 | 1 × 10−27 | 3 | 0 | 5/10 | 1/10 |
| 1Du | TrbJ; Rhizobium sp. NGR234 (NP_443810.1) | 544 | 21 | 51 | 1 × 10−06 | 14 | 0 | 5/10 | 1/10 |
| 1Bu | DNA gyrase subunit A; Campylobacter jejuni (AAS88433.1) | 348 | 99 | 99 | 1 × 10−59 | 1 | 0 | 4/10 | 0/10 |
| 1Ac | Conserved hypothetical protein; Campylobacter coli RM2228 (EAL57391.1) | 404 | 58 | 78 | 0.025 | 7 | 5 | 2/5 | 0/7 |
a
As revealed by BlastX analysis.
b
Cfv, C. fetus subsp. venerealis; Cff, C. fetus subsp. fetus.