TABLE 1.
Strains, plasmids, and cosmids used in this study
| Strain, plasmid, or cosmid | Relevant genotype, description, or properties | Reference or sourcea |
|---|---|---|
| Strains | ||
| E. coli | ||
| MC4100 | araD139 Δ(lacIZYA-argF)U169 rpsL150 relA1 flhD5301 deoC1 fruA25 rbsR22 | 14 |
| ET12567 | dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 fhuA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44 | 33 |
| DH5α | endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA96 Δ(lacIZYA-argF)U169 relA1 (φ80lacZΔM15) | Stratagene (Heidelberg, Germany) |
| XL1-Blue MR | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac | Stratagene (Heidelberg, Germany) |
| Streptomyces | ||
| ATCC 700974 | Streptomyces sp.; Nalr | ATCC |
| Tü 6 | Streptomyces griseus | DSMZ |
| Tü 17 | S. griseus ETH 10073 | Strain collection, Tübingen |
| Tü 19 | S. griseus ETH 4289 | Strain collection, Tübingen |
| M145 | Streptomyces coelicolor A3(2) SLP2− SLP3− | 27 |
| SIP1192 | ATCC 700974 ΔdhbACE Neor with a possible polar effect on downstream dhbBG genes | This study |
| SIP1194 | ATCC 700974 ΔdhbCE Neor with a possible polar effect on downstream dhbBG genes | This study |
| TK23 | Streptomyces lividans 66 spc-1 SLP2− SLP3− | 27 |
| J1074 | Streptomyces albus G | 15 |
| Plasmids | ||
| pOJ436 | acc(3)IV (Aprr) λ cos oriTRK2; φC31 integration function, ColE1 replicon | 12 |
| pSET152ermE*p | acc(3)IV (Aprr) oriTRK2; pUC18 replicon, φC31 integration function, constitutive promoter ermE*p | Stefan Pelzer (Combinature, Berlin, Germany) |
| pKC1132 | acc(3)IV (Aprr) lacZα oriTRK2; pUC18 replicon | 12 |
| pSP126/1049 | dhbAEBG in pSET152ermE*p | This study |
| pSP126/1079 | dhbACEBG in pSET152ermE*p | This study |
| pSU18 | p15A replicon; Camr, high copy number | 6 |
| pUB307 | RP1 derivative; self-transmissible oriT-mobilizing plasmid | 8 |
| Cosmids | ||
| 679 | Integrative cosmid containing orf1, orf2, and dhbACEBG griABCD in pOJ436 | This study |
| 680 | Integrative cosmid comprising orf1, orf2, dhbACEBG griABCDEFGH, andorf3 in pOJ436 | This study |
a
ATCC, American Type Culture Collection, Manassas, VA; DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.