TABLE 2.

Proposed genes and encoded proteins of the griseobactin gene locus of Streptomyces sp. strain ATCC 700974 and comparison with proteins from databases

Gene	Protein size (no. of amino acids)	Proposed function	Homologue (no. of amino acids)/organism (UniProt accession no.)	Identity, similarity (%)
orf1	547	Phosphoglucomutase	Pgm (555)/E. coli (P36938)	58, 72
orf2	422	Hypothetical protein
dhbG	79	Aryl carrier for DHBA	DhbB (312; C-terminal 88 amino acids)/B. subtilis (P45743)	38, 52
			EntB (285; C-terminal 78 amino acids)/E. coli (P0ADI4)	43, 58
dhbB	210	Isochorismatase	DhbB (312; N-terminal 210 amino acids)/B. subtilis (P45743)	54, 74
			EntB (285; N-terminal 210 amino acids)/E. coli (P0ADI4)	44, 66
dhbE	545	DHBA-AMP ligase	DhbE (539)/B. subtilis (P40871)	65, 77
			EntE (536)/E. coli (P10378)	46, 63
dhbC	401	Isochorismate synthase	DhbC (398)/B. subtilis (P45744)	52, 62
			EntC (391)/E. coli (P0AEJ2)	37, 52
dhbA	276	2,3-Dihydro-DHBA dehydrogenase	DhbA (261)/B. subtilis (P39071)	49, 64
			EntA (248)/E. coli (P15047)	45, 56
griA	779	Siderophore uptake membrane transporter	FepD, FepG (334, 330)/E. coli (P23876, P23877)	33, 49
			FhuB (660)/E. coli (P06972)	27, 42
			FhuB, FhuG (384, 336)/B. subtilis (P49936, P49937)	29, 47
griB	424	Esterase	Fes (374)/E. coli (P13039)	30, 41
griC	439	MFS exporter	HsrA (475)/E. coli (P31474)
griD	67	MbtH-like protein	MbtH (71)/Mycobacterium tuberculosis (O05821)	39, 49
griE	2,468	NRPS	DhbF (2,378)/B. subtilis (P45745)	47, 61
griF	397	Amidohydrolase	AbgB (481)/E. coli (P76052)	24, 34
griG	335	Siderophore-binding lipoprotein	FhuD (315)/B. subtilis (P37580)	27, 43
griH	263	ATPase of siderophore uptake ABC transporter	FepC (271)/E. coli (P23878)	46, 64
orf3	339	Lysine arginine ornithine transport system kinase	ArgK (331)/E. coli (P27254)	41, 57
orf4	>349	Methylmalonyl-CoA mutase large subunit	MutB (728; C-terminal 347 amino acids)/ Propionibacterium freudenreichii (P11653)	68, 79