TABLE 2.
Primers
| Primer | Sequencea | Use |
|---|---|---|
| sigA f | TCGGTTCGCGCCTACCT | TaqMan primer |
| sigA p | TTGAGCAGCGCTACCTTGCCG | TaqMan probe |
| sigA r | TGGCTAGCTCGACCTCCTCCT | TaqMan primer |
| ruvC f | GCTCAGGTCACCGCGATG | TaqMan primer |
| ruvC p | CACCAAAATCCTTGCGCTGCAAGCT | TaqMan probe |
| ruvC r | CGGCCGGTGTCGGTT | TaqMan primer |
| ruvC small f | AATCTAGAGGTCGGGGCCGGCTCAATCTC | Cloning |
| ruvC small r | CGAAGCTTAGCTGCCGACCACGCCCACTCT | Cloning and primer extension |
| ruvC 1f | CTAGGTGTCCTGCGCGGGATGTCGGGACGATCCGCTAGCGTATCGAACGATTGTTCGGAAATGG | Cloning |
| ruvC 1r | AGCTCCATTTCCGAACAATCGTTCGATACGCTAGCGGATCGTCCCGACATCCCGCGCAGGACAC | Cloning |
| ruvC 3f | CTAGGTGTCCTGCGCGGGATGTCGGGACGATCCGCTAGCGTATCGAACGA | Cloning |
| ruvC 3r | AGCTTCGTTCGATACGCTAGCGGATCGTCCCGACATCCCGCGCAGGACAC | Cloning |
| ruvC 7f | CTAGGTGTCCTGCGCGGGATGTCGGGACGATCCGCTCGCGTATCGAACGATTGTTCGGAAATGG | Cloning |
| ruvC 7r | AGCTCCATTTCCGAACAATCGTTCGATACGCGAGCGGATCGTCCCGACATCCCGCGCAGGACAC | Cloning |
| ruvC 8f | CTAGGTGTCCTGCGCGGGATGTCGGGACGATCCGCTAGCGTATCGAACGATTGTTCAGAAATGG | Cloning |
| ruvC 8r | AGCTCCATTTCTGAACAATCGTTCGATACGCTAGCGGATCGTCCCGACATCCCGCGCAGGACAC | Cloning |
a
The underlined bases indicate bases added to the native sequence to form overhangs compatible with restriction sites on annealing of appropriate pairs of oligonucleotides.