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A, effects of 300 nm TRH in the presence of 1 μm tetrodotoxin (TTX). The trace is representative of five experiments. B, effect of 300 nm TRH in the presence of 1 μm TTX and 1 μm nifedipine. C, TRH-induced depolarization in the absence of extracellular sodium. At the arrow (DC), depolarizing current was used to return the cell to its original resting potential.
