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. 2009 Oct 19;587(Pt 23):5709–5721. doi: 10.1113/jphysiol.2009.181420

Figure 5. Contribution of PLC to the TRH response.

Figure 5

A, the PLC inhibitor U-73122 elicited a membrane depolarization. The dashed line represents a membrane potential of −60 mV. The trace is representative of four experiments. B, the TRH response in the presence of U-73122 measured in voltage clamp at VH of −50 mV. U-73122 itself induced an inward current, and after this current had reached a steady level, TRH was applied. C, inhibition of the U-73122-induced current by Gd3+. Gd3+ was applied before and during application of U-73122. Horizontal dashed line shows the zero current level. D, effect of pH 6.5 solution in the presence of U-73122. E, the TRH-induced current was unaffected in the presence of U-73343, an inactive isomer of U-73312. F, inhibition of the TRH-induced current by NCDC, a PLC inhibitor. G, summary of the mean TRH-induced current in the presence of 0.5 μm U-73122, 0.5 μm U-73343, 100 μm NCDC or 10 μm edelfosine. ***P < 0.01 compared with control.