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The number of slow cycling cells was increased by LiCl. Y79 cells were incubated with LiCl and pulse-chased with BrdU for a total of 3 weeks. The number of live cells retaining BrdU was measured by flow cytometry. The percent of gated live BrdU+ cells was calculated and normalized to untreated cells and there was significantly greater BrdU+ cells in the LiCl-treated samples at a p-value<0.05, indicated by the asterisk. Each experiment was repeated three times.
