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. 2009 Dec 25;66(Pt 1):37–40. doi: 10.1107/S1744309109047708

Table 1. Purification of E. coli DAP epimerase.

One unit of enzyme activity is equal to the reduction of 1 µmol of NADP+ per second.

  Total protein (mg) Total activity (U) Specific activity (U mg−1) Degree of purification (fold)
Crude extract 504 103 0.205  
Ni2+-affinity liquid chromatography 98.7 56.7 0.574 2.80
Gel-filtration chromatography 39.2 46.1 1.18 5.76