Table 1. Purification of E. coli DAP epimerase.
One unit of enzyme activity is equal to the reduction of 1 µmol of NADP+ per second.
| Total protein (mg) | Total activity (U) | Specific activity (U mg−1) | Degree of purification (fold) | |
|---|---|---|---|---|
| Crude extract | 504 | 103 | 0.205 | |
| Ni2+-affinity liquid chromatography | 98.7 | 56.7 | 0.574 | 2.80 |
| Gel-filtration chromatography | 39.2 | 46.1 | 1.18 | 5.76 |