Table 1. Optimized crystallization conditions for native and SeMet-derivative UgdG proteins.
Crystal | Precipitant solution | Drop volume: protein + precipitant (ml) | Time (h) | Temperature (K) | Cryoprotectant | Other notes |
---|---|---|---|---|---|---|
Native | 0.1 M Na citrate pH 5.6, 20%(w/v) PEG 4K, 10%(v/v) 2-propanol | 1 + 1 | 24 | 293 | Mother liquor with 25%(v/v) glycerol | Microbatch, additive 0.05 mM NaF |
SeMet derivative | 0.1 M MES pH 6.5, 40%(w/v) PEG 200 | 1 + 1 | 24–48 | 293 | Mother liquor with 25%(v/v) PEG 400 | Vapour diffusion |