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. 2009 Dec 25;66(Pt 1):69–72. doi: 10.1107/S174430910904929X

Table 1. Optimized crystallization conditions for native and SeMet-derivative UgdG proteins.

Crystal Precipitant solution Drop volume: protein + precipitant (ml) Time (h) Temperature (K) Cryoprotectant Other notes
Native 0.1 M Na citrate pH 5.6, 20%(w/v) PEG 4K, 10%(v/v) 2-propanol 1 + 1 24 293 Mother liquor with 25%(v/v) glycerol Microbatch, additive 0.05 mM NaF
SeMet derivative 0.1 M MES pH 6.5, 40%(w/v) PEG 200 1 + 1 24–48 293 Mother liquor with 25%(v/v) PEG 400 Vapour diffusion