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. 2009 Dec 25;66(Pt 1):76–80. doi: 10.1107/S1744309109049707

Figure 3.

Figure 3

Analysis of the crystal content. (a) Protein analysis. Coomassie-stained 15% denaturing SDS–polyacrylamide gel. Lane 1, markers; lane 2, dissolved crystals; lane 3, purified Pop6–Pop7 heterodimer used in crystallization. Pop7 is known to have anomalously low mobility on SDS gels (Perederina et al., 2007 ▶). (b) RNA analysis. Ethidium bromide-stained 15% denaturing (8 M urea) polyacrylamide gel. Lane 1, markers; lane 2, dissolved crystals; lane 3, initial preparation of the P3-­domain RNA used in crystallization.