Table 1.

Effect of antisense oligonucleotides to PRKX on PMA-induced macrophage maturation of HL-60 cells

HL-60 cells treated with	Cell number, × 104 cells/ml	Adherent and spread cells, %	Phagocytic cells, %	Cells reacting with anti-MAC1 antibody, %
—	125  ±  11	<1	6  ±  3	<1
PMA	47  ±  5	72  ±  13	91  ±  5	83  ±  7
PMA + SENSE1	40  ±  12	76  ±  15	84  ±  5	77  ±  4
PMA + ANTISENSE1	117  ±  25	3  ±  1	5  ±  1	5  ±  2
PMA + SENSE2	41  ±  6	82  ±  3	79  ±  3	88  ±  3
PMA + ANTISENSE2	93  ±  16	5  ±  1	12  ±  3	<1

In the absence of the differentiation inducers, the oligonucleotides neither affected cell proliferation nor induced appearance of any maturation markers. The data represent the mean results of three independent experiments ± SD.