Table 2.

Effect of antisense oligonucleotides to PRKX on DMSO induced granulocyte maturation of HL-60 cells

HL-60 cells treated with	Cell number, ×104 cells/ml	Cells reacting with anti-MAC1 antibody, %
–	57  ±  5	<1
DMSO	7  ±  2	53  ±  4
DMSO + SENSE1	8  ±  3	49  ±  5
DMSO + ANTISENSE1	51  ±  6	11  ±  4
DMSO + SENSE2	11  ±  3	61  ±  6
DMSO + ANTISENSE2	61  ±  6	6  ±  1

In the absence of the differentiation inducers, the oligonucleotides neither affected cell proliferation nor induced appearance of any maturation markers. The data represent the mean results of three independent experiments ± SD.