Table 2.
Sequence of primers and probes used for Taqman real-time quantitative PCR
| Gene | Oligo | Sequence | Accession Number |
|---|---|---|---|
| Gapdh | Forward | 5'-GACGGCCGCATCTTCTTGT-3' | |
| Probe | JOE-5'-CAGTGCCAGCCTCGTCCCGTAGA-3'-BHQ | [EMBL:DQ403054] | |
| Reverse | 5'-CACACCGACCTTCACCATTTT-3' | ||
| 18S | Forward | 5'-GCCGCTAGAGGTGAAATTCTTG-3' | |
| Probe | VIC-5'-TCTGGTCCGTCTTGCGCCGG-3'-Tamra | [EMBL: M27358] | |
| Reverse | 5'-CATTCTTGGCAAATGCTTTCG-3' | ||
| Akr1c6 | Forward | 5'-CGTCCAGAACTCGTACGG-3' | |
| Probe | FAM-5'-TGCTTGGAACAGTCATTGAAGCAACTCCA-3'-Tamra | [EMBL: BC056643] | |
| Reverse | 5'-GAGGTACAGGTCCACATAGTCCAA-3' | ||
| Akr1c12 | Forward | 5'-TTTGTGATGTGGCCAAAAGG-3' | |
| Probe | FAM-5'-AAGCCCTGCCCTAATTGCACTTCGAT-3'-Tamra | [EMBL: BC063780] | |
| Reverse | 5'-GGCACAATCCCACGTTGAA-3' | ||
| Akr1c13 | Forward | 5'-ATCATGGCTTCTGCCTGATGT-3' | |
| Probe | FAM-5'-TGTGTGTGGACAGTGATGCTGGCAATATG-3'-Tamra | [EMBL: BC021937] | |
| Reverse | 5'-ACAAGTCCATCCAACAGTCCATCT-3' | ||
| Akr1c14 | Forward | 5'-CTACGGATGATTGTGAATCACCAT-3' | |
| Probe | FAM-5'-TGGGCACTGGGAGCCAAACCTAAGT-3'-Tamra | [EMBL: BC013482] | |
| Reverse | 5'-GGCACTTGCTGCTCTAACAGAA-3' | ||
| Akr1c18 | Forward | 5'-GCCCTTGCCACGAGTTCTATTA-3' | |
| Probe | FAM-5'-TTGTGTGATGCTGGACTCTCAGATGCC-3'-Tamra | [EMBL: BC034259] | |
| Reverse | 5'-GGAGGCGGTGTGTCGAGTT-3' | ||
| Akr1c19 | Forward | 5'-AGCAATGAGTTCCAAACAGCAA-3' | |
| Probe | FAM-5'-ATGGCAACTTCATCCCTGCGCTG-3'-Tamra | [EMBL: BC087964] | |
| Reverse | 5'-CTGGTTTGTAGGTGCCAAAGC-3' | ||
| Akr1c20 | Forward | 5'-AACGCCTGCGCTGATTG-3' | |
| Probe | FAM-5'-CCTTCGCTACCAGGTGCAACGTGG-3'-Tamra | [EMBL: BC021607] | |
| Reverse | 5'-GAAACTCTTGGCTAGGACCACAA-3' | ||
| Akr1c21 | Forward | 5'-CCATGGCAAAAAAATATAATCGAACT-3' | |
| Probe | FAM-5'-CAGCCTTGATTGCCCTTCGCTACCA-3'-Tamra | [EMBL: BC061057] | |
| Reverse | 5'-GACCACAATCCCACGCTGTA-3' |