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. 2009 Dec 15;9:101. doi: 10.1186/1472-6750-9-101

Figure 8.

Figure 8

Differentiation capacity of three different stem cell lines after five passages in USSC growth mediumACF. After five passages in USSC growth mediumACF, cells were dissociated and plated on coated plates, as described in Materials and Methods. The next day, differentiation medium was added. A, Bone differentiation cultures were stained with Alizarin Red S to test for mineral deposition. (i) USSC cultured in fetal calf serum; and (ii) cells cultured in USSC growth mediumACF for five passages. B, Epithelial differentiation was confirmed by RT-PCR of SPC of cells differentiated in Small Airway Growth Medium. Lane 1-6 USSCs passaged in fetal calf serum: Lane 1, USSC Line 1; Lane 2, RT-negative control for USSC Line 1; Lane 3, USSC Line 2; Lane 4, RT-negative control for USSC Line 2; Lane 5, USSC Line 3; and Lane 6, RT-negative control for USSC Line 3. Lanes 7-12 USSCs passaged five times in USSC growth mediumACF: Lane 7, USSC Line 1; Lane 8, RT-negative control for USSC Line 1; Lane 9, USSC Line 2; Lane 10, RT-negative control for USSC Line 2; Lane 11, USSC Line 3; and Lane 12, RT-negative control for USSC Line 3.