Table 1.

Summary of conditions and primers used for RT-PCR.

Genes	Primer Sequences (5'-3')		Cycles	Size (bp)	Annealing Temperature (°C)
Pluripotency-associated markers
Oct4	F	TCTCGCCCCCTCCAGGT	30	218	53
	R	GCCCCACTCCAACCTGG
Sox2	F	GGCAGCTACAGCATGATGCAGGACC	34	130	60
	R	CTGGTCATGGAGTTGTACTGCAGG
Endodermal-associated markers
FoxA2	F	GGGAGCGGTGAAGATGGA	38	89	55
	R	TCATGTTGCTCACGGAGGAGTA
Gata6	F	GAGTGAGAGAAGATGGAAGGG	38	250	55
	R	CGCACATGAAATCTGGATGTGG
Ectodermal-associated markers
Nestin	F	CAGCTGGCGCACCTCAAGATG	30	209	55
	R	AGGGAAGTTGGGCTCAGGACTGG
Pax6	F	AACAGACACAGCCCTCACAAACA	30	275	55
	R	CGGGAACTTGAACTGGAACTGAC
Mesodermal-associated markers
Brachyury	F	GAACCAGCACCCTGTGTCCAC	38	608	57
	R	GCCACGACAAAAAGTCACTGC
Goosecoid	F	CTCAACCAGCTGCACTGTC	38	322	55
	R	GTCAGCTGTCCGAGTCCAAATC
Control-associated markers
GAPDH	F	ATGGAGAAGGCTGGGGCTC	28	196	55
	R	AAGTTGTCATGGATGACCTTG