Figure 5.

Isolation and characterization of an LMO-4-interacting protein, mDEAF-1. (A) The amino acid sequence of mDEAF-1 compared with Drosophila DEAF-1. (B) A GST-interaction assay with the indicated GST proteins and ³⁵S-labeled mDEAF-1. (C) A GST-interaction assay with the indicated GST proteins and the following ³⁵S-labeled mDEAF-1 proteins shown schematically on top: A, full length; B, residues 1–344; C, residues 219–588. (D) In situ hybridization showing mDEAF-1 expression in dorsal root ganglia at e15.5, sagittal cut of neck region. (E) In situ hybridization showing mDEAF-1 expression in submandibular gland and epidermis at e17.5. (F) In situ hybridization showing expression of mDEAF-1 in breast epithelial cells from a 12.5-day pregnant mother.