Figure 4.

Effect of Some Other PLD Activators on Microtubules in BY-2 Cells.

(A) to (D) Confocal laser scanning micrographs of GFP-MAP4–transformed interphase cells after treatment with some known PLD activators. These images are maximum projections of 40 confocal slices covering a depth of 15 μm and denoting approximately a hemicylinder of each cell. Bars = 5 μm.

(A) Control.

(B) Osmotic stress (150 mM NaCl).

(C) Fungal elicitor xylanase (100 μg/mL).

(D) Hypoosmotic stress (1:1 dilution with distilled water).

(E) TLC analyses of lipid extracts from ³²P-prelabeled cells treated with or without the agonists noted above in the presence of 0.5% n-butanol.