Figure 5.

Effects of Microtubule-Stabilizing and -Depolymerizing Drugs on n-Butanol–Triggered Microtubule Release and PBut Formation in GFP-MAP4–Transformed BY-2 Cells.

(A) to (D) Confocal laser scanning micrographs of interphase cells preincubated with 10 μM taxol ([A] and [C]) and subsequent treatment with 0.5% n-butanol for 15 min ([B] and [D]).

(E) to (H) Confocal laser scanning micrographs of interphase cells preincubated with 10 μM oryzalin to depolymerize the microtubules ([E] and [G]) and subsequent treatment with 0.5% n-butanol for 15 min ([F] and [H]).

(A), (B), (E), and (F) show maximum projections of 40 confocal slices covering a depth of 15 μm and denoting approximately a hemicylinder of each cell. (C), (D), (G), and (H) show xz cross-sections of the cells shown in (A), (B), (E), and (F), respectively. Bars = 5 μm.

(I) TLC analyses of lipid extracts from ³²P-prelabeled and taxol- or oryzalin-pretreated or untreated BY-2 cells with 15 min of incubation in 0.5% n-butanol.