Figure 2. Transcripts level of TaVRN-B2 and TaVRN-A2 in both spring and winter wheat during cold acclimation.

A) TaVRN-B2 and TaVRN-A2, in cold acclimated plants. Wheat plants were grown for 14 days at 20°C under a long day (16 h) photoperiod, transferred to 4°C under identical photoperiods, and then sampled at regular intervals. B) TaVRN-B2 in cold acclimated plants under short day and long day conditions. Winter wheat plants were grown for 14 days at 20°C under either a long day (16 h) or a short day (8 h) photoperiod, transferred to 4°C under identical photoperiods, and then sampled at regular intervals. qRT-PCR were done using total reverse-transcribed RNA isolated from wheat aerial part. C) TaVRN-B2 relative transcripts abundance in different tissues. Winter wheat were grown for 7 days at 20°C. Non-acclimated control plants (NA) were maintained at 20°C for 6 days. Cold-acclimated plants (CA) were transferred at 4°C for 36 days. Total RNA was isolated from leaves, crown and roots, reverse-transcribed and subjected to qRT-PCR. Data shown represent mean values obtained from independent amplification reactions (n = 4), and the error bars indicate the range of possible RQ values define by the SE of the delta threshold cycles (Cts). Experiment was repeated three times with similar results.