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. 2010 Jan 13;5(1):e8690. doi: 10.1371/journal.pone.0008690

Figure 6. TaVRN-B2 enhances freezing tolerance in Arabidopsis.

Figure 6

A) Control and transgenic plants after freezing test. Plants were grown for 3 weeks at 20°C (NA) or grown for 3 weeks at 20°C then transferred to 4°C for 7 days (CA7). Controls plants wild type (WT) and 35S::GUS), transgenic 35S::TaVRN-B2 lines (Line1–Line3). Plants were subjected to freezing test (NA frozen to −6.5°C and CA7 frozen to −10.5°C). Pictures were captured for the same plants before the freezing and after a recovery period of 2 weeks. B) Survival rate after freezing stress expressed as a percent of surviving plants. A statistical difference measurements from three independent experiments (n = 33). The threshold for statistical significance is indicated by an asterisk on top of each histogram columns; comparison between GUS and the three LINES; *: P<0.05, **: P<0.01, ns: P>0.05.