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Coefficients of variation for canine haemoplasma qPCR assays—based on calculated copy number (and threshold cycle). Mhc: Mycoplasma haemocanis; CMhp: “Candidatus Mycoplasma haematoparvum”.
| Assay | Mhc | CMhp |
|---|---|---|
| Intra-assay—high [plasmid] | 9.2% (0.87%) | 9.2% (0.79%) |
| Intra-assay—low [plasmid] | 19.8% (0.98%) | 17.6% (0.86%) |
| Inter-assay—high [plasmid] | 4.7% (0.43%) | 5.1% (0.40%) |
| Inter-assay—low [plasmid] | 2.8% (0.13%) | 13.5% (0.66%) |
