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. 2009 Jul 31;95(1):71–78. doi: 10.3324/haematol.2009.009811

Figure 2.

Figure 2.

Internalization of CLL-1 upon monoclonal antibody binding. (A) HEK293 cells stably expressing CLL-1 were stained with fluorescently labeled CLL-1 mouse monoclonal antibody 1075.7, incubated for the indicated times at 37°C, fixed and examined using fluorescence microscopy. (B) CLL-1 internalization in HL-60 cells. After staining with monoclonal antibody 1075.7, cells were incubated for the indicated times at 37°C after which they were (squares) or were not (diamonds) subjected to protease treatment prior to flow cytometry analysis. The staining observed in the protease-treated cells represents only intracellular staining.