Figure 2.

NFKBIA and TNFAIP3 sequences of 20 pooled LP and non-tumor cells of primary NLPHL. (Left) Electropherograms showing a, presumably heterozygous, single nucleotide replacement mutation in exon 5 of the NFKBIA gene present in LP but not in non-tumor cells from a primary NLPHL (case 3). Additional sequencing of single LP cells further support a heterozygous mutation (Table 1). (Right). Electropherograms showing a 2 bp insertion in exon 9 of the TNFAIP3 gene present in LP but not in non-tumor cells from a primary NLPHL (case 9). Additional sequencing of single LP cells and immunophenotyping and interphase cytogenetics (FICTION) analysis indicate either a subclonal heterozygous mutation or a heterozygous mutation plus a copy number gain of the wild-type allele only in LP cells (Table 1). Positions affected by mutations in LP cells and their equivalent in non-tumor cells are indicated by arrows.