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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: FEBS J. 2009 Oct 27;276(23):6992–7005. doi: 10.1111/j.1742-4658.2009.07407.x

Fig. 3. WT Cx43-eYFP is selectively targeted to the basolateral domain of MDCK cells.

Fig. 3

A,B,C. Three independent MDCK clones expressing WT Cx43-eYFP were cultured on filter inserts. Z sections of cell monolayers are shown in the top panels. Lines drawn through the XY plane in the bottom panels indicate the location of the Z sections. WT Cx43-eYFP was expressed on the basolateral membrane domain of MDCK cells. D. Z section of monolayer of uninfected MDCK cells showed background fluorescence. E. Z section of monolayer of MDCK cells expressing only eGFP showed a diffuse cytoplasmic pattern. Scale bar, 10 um.