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. Author manuscript; available in PMC: 2010 Dec 29.
Published in final edited form as: Curr Biol. 2009 Dec 3;19(24):2086–2090. doi: 10.1016/j.cub.2009.10.051

Figure 1. Laser axotomy and imaging of peripheral axon regeneration.

Figure 1

(A) Experimental design. Dorsal view of zebrafish embryo head; eyes are grey, trigeminal axons are green. Arrowhead indicates site of axotomy in all panels. Axon regeneration was monitored by time lapse for ≥ 12 hours and reinnervation of the denervated territory (yellow shading) by new axon growth (red) was calculated. (B) Dorsal and lateral views of a trigeminal axon reconstructed in 3-D. The axon arborizes within a mostly 2-D plane. (C–G) Time series of confocal image stacks. Same axon as in 1B. Time stamps are in minutes relative to axotomy at 30 hpf. Olive shading highlights the denervated region. Scale bar = 50 μm. See Movie S1.