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. Author manuscript; available in PMC: 2010 Dec 14.
Published in final edited form as: Curr Biol. 2009 Nov 12;19(23):2026–2030. doi: 10.1016/j.cub.2009.10.020

Figure 4.

Figure 4

Relationship between the timing of spindle disassembly and either the passage of one SPB into the bud or the loss of microtubules from the bud neck. “No checkpoint” histogram depicts the time elapsed between the arrival of one pole of an elongating anaphase spindle in the bud and subsequent spindle disassembly in 132 cells with properly aligned spindles that do not exhibit checkpoint-dependent delay; from movies of GFP-tubulin collected on a confocal microscope (20.3 ± 3.6 min; mean ± SD). Dashed lines indicate the 95% confidence interval (mean ± 2SD). “Checkpoint failure after microtubule loss” depicts the time elapsed between the ablation of neck-interacting microtubules and spindle disassembly within the mother compartment in 17 cells. “Checkpoint satisfaction” depicts the time between the arrival of one spindle pole in the bud and spindle disassembly in cells that correct spindle alignment defects after checkpoint-dependent anaphase delay; from movies collected on a confocal microscope of 52 cells expressing GFP-tubulin (21.1 ± 9.1 min; mean ± SD). Dashed lines indicate the 95% confidence interval. Scales of the Y-axes are not equivalent. Strain: yJC5603.