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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: FEBS J. 2009 Nov 18;277(1):119–127. doi: 10.1111/j.1742-4658.2009.07458.x

Figure 2.

Figure 2

LF-K518E/E682G is defective at killing RAW 264.7 cells and inducing the Nlrp1b inflammasome. (A) PA and various concentrations of wild-type LF (σ) or LF-K518E/E682G (○) were incubated with RAW 264.7 cells and viability was assessed after 4 h using the MTS assay. Values represent the mean ± standard error of the mean for three independent experiments. (B) RAW 264.7 cells were left untreated (black bars) or treated with PA and wild-type LF (white bars) or PA and LF-K518E/E682G (grey bars) for 4 h or 24 h. Viability was assessed as the fraction of cells that excluded trypan blue. Values represent the mean ± standard error of the mean for three independent experiments. (C) HT1080 cells were mock transfected or were transfected with plasmids encoding Nlrp1b, pro-caspase-1 and pro-IL-1b. After 24 h, cells were treated with PA and either wild-type LF or LF-K518E/E682G. IL-1β, MAPKK1, and β-actin were detected by immunoblotting. The results shown represent three independent experiments.