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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: FEBS J. 2009 Nov 18;277(1):119–127. doi: 10.1111/j.1742-4658.2009.07458.x

Figure 5.

Figure 5

LF-K518E/E682G causes death of melanoma cells. (A) PA and various concentrations of wild-type LF (σ) or LF-K518E/E682G (○) were incubated with MALME-3M cells and viability was assessed after 72 h using the MTS assay. Values represent the mean ± standard error of the mean for three independent experiments. (B) MALME-3M cells were treated with 10−8 M PA and the indicated concentrations of either wild-type LF or LF-K518E/E682G for 2 h and then treated with 2.5 μg/mL anisomycin for 15 min. Cellular lysates were prepared and probed for phosphorylated MAPKs. Values indicate the level of phosphorylated MAPK in toxin-treated cells as a fraction of the level in cells that were not treated with toxin. The results shown represent the mean ± standard error of the mean for three independent experiments.