Males of the indicated genotypes were trained for 1 hr and tested at varyious times after training. The training condition is indicated above each panel, and the tester type is indicated below each panel (mV, mature virgin; iV, immature virgin; -d, decapitated). Data are presented as means ± SEM.
(A) dnc1 males were trained and tested for memory immediately after training. These males failed to form trainer-specific memory and suppressed courtship of a mature virgin tester equally regardless of training condition (p > 0.05).
(B) amn28A males were trained and tested for memory immediately after training. They were able to form trainer-specific memory with either a mature virgin trainer (***p < 0.001, for comparison to the null hypothesis) or a pheromone filter and immature-virgin courtship object (**p < 0.05, for comparison to the null hypothesis).
(C) amn28A males failed to maintain memory. Memory retention [(1 − CItest/mCIsham)/m(1 − CItest/mCIsham at t = 0)] is plotted against time after training. Mutant male memory decayed to zero by 1.5 hr, whereas wild-type memory was robust out to 2.5 hr (***p < 0.001 for comparison between wild-type and amn28A).