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MDA-MB-231 tumours result in pulmonary metastases, which express the hypoxia factors HIF1α and GLUT1, as well as the anoxia factors ATF3 and ATF4. (A) Lungs of xenograft-bearing mice demonstrated metastatic foci, as indicated by the solid arrows on haematoxylin & eosin (H&E) slides. Lungs from control mice did not contain any tumours ( × 100 magnification). (B) Expression of HIF1α, GLUT1, ATF3, and ATF4 in tumour cells in pulmonary metastatic foci.
