Figure 3.

Galectin‐1‐targeted siRNA induces both a decrease of integrin‐β1 at the edges of the cell membrane and an increase in the intracellular accumulation of integrin‐β1. A. U87 control wild‐type cells (Aa,Ab) and U87 cells containing stably transfected antisense galectin‐1 vectors (Ac) were co‐stained under non‐permeabilized conditions for integrin‐β1 [the red staining at the tip of actin stress fibers (in green fluorescence)], fibrillar actin (green) and globular actin (red staining inside the cells). Ab. Higher magnification of the control wild‐type (Aa) image. B. U87 control wild‐type cells (Ba) and U87 cells containing stably transfected antisense galectin‐1 vectors (Bb) were stained under permeabilized conditions for integrin‐β1 only (in green), without any staining to reveal actin (Ba,Bb). C. U87 and Hs683 cells were transiently transfected with either scrambled siRNA (scr) or siRNA targeted against galectin‐1 (siGal1). Cells were stained for integrin‐β1 (in red) under both permeablized and non‐permeabilized conditions on either day 5 post‐transfection (Hs683) or day 7 post‐transfection (U87).