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. 2010 Jan;12(1):51–60. doi: 10.1593/neo.91272

Figure 1.

Figure 1

Gonadotropin stimulation regulates HA synthesis and degradation in ovarian carcinoma. MLS and ES2 cells were treated with hLH or hFSH (1 ng/ml) after 6, 12, 18, 24, and 48 hours or after 6, 12, 18, 24, 30, and 36 hours respectively. Cells were harvested, and total RNA was isolated and reverse-transcribed. HAS2, HAS3, Hyal1, and Hyal2 levels were determined by semiquantitative PCR. Band intensity was normalized to GAPDH. (A) Representative agarose gels of HAS2, HAS3, and GAPDH expression in MLS cells after FSH stimulation. (B) Representative agarose gels of HAS2, HAS3, and GAPDH expression in MLS cells after LH stimulation. (C) hFSH's effects on HAS2 and HAS3 expression levels at 1 ng/ml in MLS cells (error bars, SE; *P < .05, two-tailed, equal-variance t test). (D) hLH's effects on HAS2 and HAS3 expression levels at 1 ng/ml in MLS cells (error bars, SE; *P < .05, two-tailed, equal-variance t test). (E) hFSH's effect on HAS2 and HAS3 expression levels at 1 ng/ml in ES2 cells (error bars, SE; *P < .05, two-tailed, equal-variance t test). (F) hLH's effect on HAS2 and HAS3 expression levels at 1 ng/ml in ES2 cells (error bars, SE; *P < .05, two-tailed, equal-variance t test). (G) hFSH's effect on Hyal1 and Hyal2 expression levels at 1 ng/ml in MLS cells (error bars, SE; *P < .05, two-tailed, equal-variance t test). (H) hLH's effect on Hyal1 and Hyal2 expression levels at 1 ng/ml in MLS cells (error bars, SE; *P < .05, two-tailed, equal-variance t test). All t tests were compared with time 0.