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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Biopolymers. 2010 Mar;93(3):237–251. doi: 10.1002/bip.21319

Figure 11.

Figure 11

Demonstration of Homogeneous folding of A25T TTR captured on GroEL beads: In Panel A, an A25T TTR sample aggregated upon storage (EM micrograph). The accompanying SEC HPLC chromatogram indicates degree of heterogeneity and lack of native tetramer species in the aggregated sample. The aggregated were disrupted and allowed to bind onto GroEL immobilized on a sepharose bead support system (GE Healthcare). The A25T TTR was released and refolded back into its tetrameric form as evidenced by the recovery of matching HPLC SEC retention times and second derivative UV-visible spectroscopy with wild type TTR tetramers. The refolded species was homogeneous. The refolding yield for this particular experiment was ~ 70%.