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. Author manuscript; available in PMC: 2011 Mar 1.

Published in final edited form as: Biopolymers. 2010 Mar;93(3):237–251. doi: 10.1002/bip.21319

The effect of the GroEL chaperonin on 1SS-α-LA fibril/aggregate formation when added after the fibrillation/aggregation process has been initiated by incubation. Test samples of 1SS-α-LA (1mg/ml) were incubated at 37°C with stirring in sample buffer containing 10mM Mg⁺² at pH 7.4. An equimolar concentration of GroEL was added to separate test samples of 1SS-α-LA at 1 hour (), 6 hours (□), 10 hours (), and 17.5 hours () after initiation of the fibrillation/aggregation process by incubation had begun. A control sample of aggregating 1SS-α-LA (■) was also tested and plotted as a comparison. Fibril/aggregate formation was monitored by an increase in thioflavin T (ThT) fluorescence intensity at 482nm and the symbols represent ThT fluorescence intensities determined experimentally. Error (average standard deviation of the mean) was ± 0.212 for the ThT intensity measurements in this experiment. ThT fluorescence intensity data is in arbitrary units and the lines were fitted to sigmoidal curves using Origin software.

Inline graphic — The effect of the GroEL chaperonin on 1SS-α-LA fibril/aggregate formation when added after the fibrillation/aggregation process has been initiated by incubation. Test samples of 1SS-α-LA (1mg/ml) were incubated at 37°C with stirring in sample buffer containing 10mM Mg⁺² at pH 7.4. An equimolar concentration of GroEL was added to separate test samples of 1SS-α-LA at 1 hour (), 6 hours (□), 10 hours (), and 17.5 hours () after initiation of the fibrillation/aggregation process by incubation had begun. A control sample of aggregating 1SS-α-LA (■) was also tested and plotted as a comparison. Fibril/aggregate formation was monitored by an increase in thioflavin T (ThT) fluorescence intensity at 482nm and the symbols represent ThT fluorescence intensities determined experimentally. Error (average standard deviation of the mean) was ± 0.212 for the ThT intensity measurements in this experiment. ThT fluorescence intensity data is in arbitrary units and the lines were fitted to sigmoidal curves using Origin software.